Hydrophilic Interaction Chromatography
نویسندگان
چکیده
Hydrophilic interaction chromatography (HILIC) was first introduced by Alpert in 1990 [...]
منابع مشابه
Hydrophilic interaction chromatography
In the early 1990s, hydrophilic interaction (liquid) chromatography (HILIC) emerged as a new separation method. HILIC is becoming increasingly popular for the separation of polar compounds in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). It has become important for the separation of pharmaceuticals, neurotransmitters, nucleosides, nucleotides and other compounds...
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In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
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Hydrophilic interaction chromatography (HILIC) is a technique that has become increasingly popular for the separation of polar, hydrophilic, and ionizable compounds, which are diffi cult to separate by reversed phase chromatography (RP) due to their poor retention when RP is used. HILIC typically uses a polar stationary phase such as bare silica or a polar bonded phase, together with an eluent ...
متن کاملAn arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography.
An arginine-functionalized stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared for the first time by clicking arginine onto silica gel. It offers an efficient separation of organic acids, nucleotides and sugars. More interestingly, it exhibited excellent selectivity and enrichment toward acidic glycopeptides, even at a ratio of 1 : 150 to non-glycopeptides.
متن کاملPerformance of charged aerosol detection with hydrophilic interaction chromatography.
The performance of the charged aerosol detector (CAD) was investigated using a diverse set of 29 solutes, including acids, bases and neutrals, over a range of mobile phase compositions, particularly with regard to its suitability for use in hydrophilic interaction chromatography (HILIC). Flow injection analysis was employed as a rapid method to study detector performance. CAD response was 'quas...
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ژورنال
عنوان ژورنال: Separations
سال: 2023
ISSN: ['2297-8739']
DOI: https://doi.org/10.3390/separations10020120